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Article

One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications.

Molecular and cellular probes - 1 Feb 2021

Lee Nan-Ee, Ha Dae-In, Ko Jeong-Heon, Kim Yong-Sam

Abstract excerpt

Base editors and prime editors induce precise DNA modifications over one or several nucleotides in eukaryotic cells. The T7E1 assay has been widely adopted for the assessment of genome editing, but it has several limitations in the applications for prime editing and base editing due to low sensitivity, inaccuracy and additional disadvantages. Here, we propose a short inner primer-assisted, tetra primer-paired...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 33245992
DOI: 10.1016/j.mcp.2020.101675

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