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Article

Effects of reverse genetic mutations on the spectral and photochemical behavior of a photoactivatable fluorescent protein PAiRFP1.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 5 Mar 2020

Hassan Fakhrul, Khan Faez Iqbal, Song Honghong, Lai Dakun, Juan Feng

Abstract excerpt

Bacteriophytochrome photoreceptors (BphPs) containing biliverdin (BV) have great potential for the development of genetically engineered near-infrared fluorescent proteins (NIR FPs). We investigated a photoactivatable fluorescent protein PAiRFP1, was engineered through directed molecular evolution. The coexistence of both red light absorbing (Pr) and far-red light absorbing (Pfr) states in dark is essential for...

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PMID: 31806482
DOI: 10.1016/j.saa.2019.117807

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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