Article
A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein.
Nature methods - 1 Sept 2016
Rodriguez Erik A, Tran Geraldine N, Gross Larry A, Crisp Jessica L, Shu Xiaokun, Lin John Y, Tsien Roger Y
Abstract excerpt
Far-red fluorescent proteins (FPs) are desirable for in vivo imaging because with these molecules less light is scattered, absorbed, or re-emitted by endogenous biomolecules compared with cyan, green, yellow, and orange FPs. We developed a new class of FP from an allophycocyanin α-subunit (APCα). Native APC requires a lyase to incorporate phycocyanobilin. The evolved FP, which we named small ultra-red FP...
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