Incidental mutations occur frequently during transposon mutagenesis.

doi:10.1093/femsle/fnw293

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Article

Incidental mutations occur frequently during transposon mutagenesis.

FEMS microbiology letters - 1 Feb 2017

Liu Guoqiang, Kong Yingying, Liu Hualin, Peng Donghai, Ruan Lifang, Wu Yiming, Sun Ming

Abstract excerpt

Transposon mutagenesis and subsequent phenotype-driven screening have been extensively used to annotate gene function and uncover the mechanisms of biological phenomena. During this process, the resulting phenotypic differences are specifically attributable to the disrupted genes. In this study, the complementation of the mini-Tn10 transposon-disrupted gene gntP did not restore the germination ability of the...

Topics

Bacillus thuringiensis
Chromosome Mapping
DNA Transposable Elements
Escherichia coli
Gene Library
Genetic Complementation Test
Genotype
Mutagenesis, Insertional
Mutation
Phenotype
Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 28011697
DOI: 10.1093/femsle/fnw293

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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