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Article

Failure to Genotype: A Cautionary Note on an Elusive loxP Sequence.

PloS one - 1 Jan 2016

Kreuger Johan, O'Callaghan Paul

Abstract excerpt

Here we report on a technical difficulty we encountered while optimizing genotyping strategies to identify mice derived from Exoc3l2tm1a(KOMP)Wtsi embryonic stem cells obtained from the Knockout Mouse Project Repository. The Exoc3l2tm1a(KOMP)Wtsi construct encodes a "knockout-first" design with loxP sites that confer conditional potential (KO1st). We designed primers that targeted wild-type sequences flanking the...

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Identifiers

PMID: 27768725
DOI: 10.1371/journal.pone.0165012
PMCID: PMC5074582

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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