Article
High production of a class III lantipeptide AmfS in Streptomyces griseus.
Bioscience, biotechnology, and biochemistry - 1 Jan 2017
Takano Hideaki, Matsui Yuhei, Nomura Junpei, Fujimoto Masahiro, Katsumata Naoto, Koyama Takafumi, Mizuno Isamu, Amano Shoichi, Shiratori-Takano Hatsumi, Komatsu Mamoru, Ikeda Haruo, Ueda Kenji
Abstract excerpt
AmfS, a class III lantipeptide serves as a morphogen in Streptomyces griseus. Here, we constructed a high production system of AmfS in S. griseus. We isolated S. griseus Grd1 strain defective in glucose repression of aerial mycelium formation and found it suitable for the overproduction of AmfS. Two expression vectors carrying the strong and constitutive ermE2 promoter were constructed using a multicopy number...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Genetic Engineering
- Genetic Vectors
- Multigene Family
- Mutation
- Streptomyces griseus