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Article

A FACS-based screening strategy to assess sequence-specific RNA-binding of Pumilio protein variants in E. coli.

Biological chemistry - 1 Jan 2017

Kellermann Stefanie J, Rentmeister Andrea

Abstract excerpt

Sequence-specific and programmable binding of proteins to RNA bears the potential to detect and manipulate target RNAs. Applications include analysis of subcellular RNA localization or post-transcriptional regulation but require sequence-specificity to be readily adjustable to any target RNA. The Pumilio homology domain binds an eight nucleotide target sequence in a predictable manner allowing for rational design...

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Identifiers

PMID: 27682713
DOI: 10.1515/hsz-2016-0214

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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