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Article

Massive functional mapping of a 5'-UTR by saturation mutagenesis, phenotypic sorting and deep sequencing.

Nucleic acids research - 1 Jul 2013

Holmqvist Erik, Reimegård Johan, Wagner E Gerhart H

Abstract excerpt

We present here a method that enables functional screening of large number of mutations in a single experiment through the combination of random mutagenesis, phenotypic cell sorting and high-throughput sequencing. As a test case, we studied post-transcriptional gene regulation of the bacterial csgD messenger RNA, which is regulated by a small RNA (sRNA). A 109 bp sequence within the csgD 5'-UTR, containing all...

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Identifiers

PMID: 23609548
DOI: 10.1093/nar/gkt267
PMCID: PMC3695526

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Source receipt: pubmed, retrieved 7 Sept 2026.

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