Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4.

Journal of bioscience and bioengineering - 1 Mar 2012

Sano Sotaro, Yamada Yosuke, Shinkawa Tomoki, Kato Satoru, Okada Takashi, Higashibata Hiroki, Fujiwara Shinsuke

Abstract excerpt

Family A DNA polymerase (K4PolI) from Thermotoga petrophila K4 was obtained as a recombinant form, and the enzyme characteristics were analyzed. K4PolI showed thermostable DNA-dependent DNA polymerase activity with 3'-5' exonuclease activity but no detectable RNA-dependent DNA polymerase activity. Its tertiary structure was speculated by in silico modeling to understand the binding situation between K4PolI and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA-Directed DNA Polymerase
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Exonucleases

Share to Molecular Sequence Data

Share to Mutation

Share to Recombinant Proteins

Share to RNA-Directed DNA Polymerase

Share to RNA, Ribosomal, 16S

Source

Identifiers

PMID: 22143068
DOI: 10.1016/j.jbiosc.2011.11.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4.PMID 22143068Read ↗

Engineering a Thermostable Reverse Transcriptase for RT-PCR Through Rational Design of Pyrococcus furiosus DNA Polymerase.Semantic proximity Read ↗

Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases.Semantic proximity Read ↗

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication.Semantic proximity Read ↗

The expanding polymerase universe.Semantic proximity Read ↗

Single-residue engineering of Klenow fragment enables broad acceptance of chemically modified nucleotides.Semantic proximity Read ↗

Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide.Semantic proximity Read ↗

Directed polymerase evolution.Semantic proximity Read ↗

Chemical biology of DNA polymerases: from selectivity to new functions.Semantic proximity Read ↗

Improved architectures for flexible DNA production using retrons across kingdoms of lifeSemantic proximity Read ↗

Enhancing the processivity of a family B-type DNA polymerase of Thermococcus onnurineus and application to long PCR.Semantic proximity Read ↗

Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases.Semantic proximity Read ↗

PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A motif.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.