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Circular permutation engineering improves amplification efficiency of Bst DNA polymerase.

International journal of biological macromolecules - 1 Mar 2026

Xiang Rong, Liu GuangYi, Wang YanRu, Hou Yi, Hu Song-Qing

Abstract excerpt

Previously, a chimeric Bst DNA polymerase (HpStBL) with high inhibitor tolerance and reverse transcriptase activity, was constructed by introducing Hp47-Sto7d domain. However, the complex primer design and the possibility of nonspecific amplification in loop-mediated isothermal amplification (LAMP) partly restricted the broader application of HpStBL in nucleic acid detection. In order to explore the polymerase...

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PMID: 41687989
DOI: 10.1016/j.ijbiomac.2026.150825

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