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Article

Clinical Application of Picodroplet Digital PCR Technology for Rapid Detection of EGFR T790M in Next-Generation Sequencing Libraries and DNA from Limited Tumor Samples.

The Journal of molecular diagnostics : JMD - 1 Nov 2016

Borsu Laetitia, Intrieri Julie, Thampi Linta, Yu Helena, Riely Gregory, Nafa Khedoudja, Chandramohan Raghu, Ladanyi Marc, Arcila Maria E

Abstract excerpt

Although next-generation sequencing (NGS) is a robust technology for comprehensive assessment of EGFR-mutant lung adenocarcinomas with acquired resistance to tyrosine kinase inhibitors, it may not provide sufficiently rapid and sensitive detection of the EGFR T790M mutation, the most clinically relevant resistance biomarker. Here, we describe a digital PCR (dPCR) assay for rapid T790M detection on aliquots of NGS...

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Identifiers

PMID: 27631691
DOI: 10.1016/j.jmoldx.2016.07.004
PMCID: PMC5807920

Genes

EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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