Article
Simultaneous quantitation and genotyping of hepatitis B virus by real-time PCR and melting curve analysis.
Journal of virological methods - 15 Sept 2004
Payungporn Sunchai, Tangkijvanich Pisit, Jantaradsamee Pojchanad, Theamboonlers Apiradee, Poovorawan Yong
Abstract excerpt
Hepatitis B virus (HBV) genotype and HBV DNA levels have been implicated in clinical evaluation and prognosis of patients with chronic HBV infection. The aim of the present study was to develop a rapid and sensitive method for simultaneous HBV DNA quantitation and differentiation between HBV genotypes B and C in a single-step reaction by real-time PCR and melting curve analysis using SYBR Green I fluorescent dye....
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