Article
Ultrasensitive amplification refractory mutation system real-time PCR (ARMS RT-PCR) assay for detection of minority hepatitis B virus-resistant strains in the era of personalized medicine.
Journal of clinical microbiology - 1 Sept 2013
Ntziora Fotinie, Paraskevis Dimitrios, Haida Catherine, Manesis Emanuel, Papatheodoridis George, Manolakopoulos Spilios, Elefsiniotis Ioannis, Karamitros Timokratis, Vassilakis Alexis, Hatzakis Angelos
Abstract excerpt
Resistance to antiviral treatment for chronic hepatitis B virus (HBV) has been associated with mutations in the HBV polymerase region. This study aimed at developing an ultrasensitive method for quantifying viral populations with all major HBV resistance-associated mutations, combining the amplification refractory mutation system real-time PCR (ARMS RT-PCR) with a molecular beacon using a LightCycler. The...
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