Article
A novel arrangement of zinc finger nuclease system for in vivo targeted genome engineering: the tomato LEC1-LIKE4 gene case.
Plant cell reports - 1 Nov 2016
Hilioti Zoe, Ganopoulos Ioannis, Ajith Sabna, Bossis Ioannis, Tsaftaris Athanasios
Abstract excerpt
KEY MESSAGE: A selection-free, highly efficient targeted mutagenesis approach based on a novel ZFN monomer arrangement for genome engineering in tomato reveals plant trait modifications. How to achieve precise gene targeting in plants and especially in crops remains a long-sought goal for elucidating gene function and advancing molecular breeding. To address this issue, zinc finger nuclease (ZFN)-based technology...
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