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Article

Building a thermostable membrane protein.

The Journal of biological chemistry - 10 Mar 2000

Zhou Y, Bowie J U

Abstract excerpt

The poor stability of membrane proteins in detergent solution is one of the main technical barriers to their structural and functional characterization. Here we describe a solution to this problem for diacylglycerol kinase (DGK), an integral membrane protein from Escherichia coli. Twelve enhanced stability mutants of DGK were obtained using a simple screen. Four of the mutations were combined to create a...

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Source

Identifiers

PMID: 10702260
DOI: 10.1074/jbc.275.10.6975

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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