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Article

Haplotyping the human leukocyte antigen system from single chromosomes.

Scientific reports - 27 Jul 2016

Murphy Nicholas M, Burton Matthew, Powell David R, Rossello Fernando J, Cooper Don, Chopra Abha, Hsieh Ming Je, Sayer David C, Gordon Lavinia, Pertile Mark D, Tait Brian D, Irving Helen R, Pouton Colin W

Abstract excerpt

We describe a method for determining the parental HLA haplotypes of a single individual without recourse to conventional segregation genetics. Blood samples were cultured to identify and sort chromosome 6 by bivariate flow cytometry. Single chromosome 6 amplification products were confirmed with a single nucleotide polymorphism (SNP) array and verified by deep sequencing to enable assignment of both alleles at...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 27461731
DOI: 10.1038/srep30381
PMCID: PMC4961964

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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