Article
Concurrent whole-genome haplotyping and copy-number profiling of single cells.
American journal of human genetics - 4 Jun 2015
Zamani Esteki Masoud, Dimitriadou Eftychia, Mateiu Ligia, Melotte Cindy, Van der Aa Niels, Kumar Parveen, Das Rakhi, Theunis Koen, Cheng Jiqiu, Legius Eric, Moreau Yves, Debrock Sophie, D'Hooghe Thomas, Verdyck Pieter, De Rycke Martine, Sermon Karen, Vermeesch Joris R, Voet Thierry
Abstract excerpt
Methods for haplotyping and DNA copy-number typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell's alleles. As a consequence, haplotyping methods...
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