Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons.

mBio - 12 May 2015

Wetmore Kelly M, Price Morgan N, Waters Robert J, Lamson Jacob S, He Jennifer, Hoover Cindi A, Blow Matthew J, Bristow James, Butland Gareth, Arkin Adam P, Deutschbauer Adam

Abstract excerpt

UNLABELLED: Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. Here, we present a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Barcoding, Taxonomic
Share to DNA Transposable Elements

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Library

Share to Genetic Fitness

Share to Mutagenesis, Insertional

Share to Pseudomonas

Share to Reproducibility of Results

Share to Shewanella

Source

Open in PubMed Open full text

Identifiers

PMID: 25968644
DOI: 10.1128/mBio.00306-15
PMCID: PMC4436071

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons.PMID 25968644Read ↗

Dual transposon sequencing (Dual Tn-seq) to probe genome-wide genetic interactionsSemantic proximity Read ↗

Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Semantic proximity Read ↗

Identifying microbial fitness determinants by insertion sequencing using genome-wide transposon mutant libraries.Semantic proximity Read ↗

Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions.Semantic proximity Read ↗

Implementation and Data Analysis of Tn-seq, Whole-Genome Resequencing, and Single-Molecule Real-Time Sequencing for Bacterial Genetics.Semantic proximity Read ↗

Mutant phenotypes for thousands of bacterial genes of unknown function.Semantic proximity Read ↗

A novel papillation assay for the identification of genes affecting mutation rate in Pseudomonas putida and other pseudomonads.Semantic proximity Read ↗

Sequence-verified two-allele transposon mutant library for Pseudomonas aeruginosa PAO1.Semantic proximity Read ↗

Signature-tagged mutagenesis: barcoding mutants for genome-wide screens.Semantic proximity Read ↗

Mutualism reduces the severity of gene disruptions in predictable ways across microbial communitiesSemantic proximity Read ↗

Transposon insertion sequencing reveals novel hypermutator genes in Acinetobacter baumannii.Semantic proximity Read ↗

Transposon Insertion Sequencing, a Global Measure of Gene Function.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.