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Article

Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system.

Nucleic acids research - 14 Oct 2016

Singh Atul K, Carette Xavier, Potluri Lakshmi-Prasad, Sharp Jared D, Xu Ranfei, Prisic Sladjana, Husson Robert N

Abstract excerpt

Despite many methodological advances that have facilitated investigation of Mycobacterium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen remains difficult. Here, we describe an optimized CRISPR-based method to inhibit expression of essential genes based on the inducible expression of an enzymatically inactive Cas9 protein together with gene-specific guide RNAs (CRISPR...

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Identifiers

PMID: 27407107
DOI: 10.1093/nar/gkw625
PMCID: PMC5062980

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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