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Article

Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin.

Scientific reports - 6 Jul 2016

Ahmad Shoeb, Pecqueur Ludovic, Dreier Birgit, Hamdane Djemel, Aumont-Nicaise Magali, Plückthun Andreas, Knossow Marcel, Gigant Benoît

Abstract excerpt

Affinity maturation by random mutagenesis and selection is an established technique to make binding molecules more suitable for applications in biomedical research, diagnostics and therapy. Here we identified an unexpected novel mechanism of affinity increase upon in vitro evolution of a tubulin-specific designed ankyrin repeat protein (DARPin). Structural analysis indicated that in the progenitor DARPin the...

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Identifiers

PMID: 27380724
DOI: 10.1038/srep28922
PMCID: PMC4933879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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