Thermostable designed ankyrin repeat proteins (DARPins) as building blocks for innovative drugs

doi:10.1016/j.jbc.2021.101403

Article

Thermostable designed ankyrin repeat proteins (DARPins) as building blocks for innovative drugs

15 Nov 2021

Abstract excerpt

Designed ankyrin repeat proteins (DARPins) are antibody mimetics with high and mostly unexplored potential in drug development. By using in silico analysis and a rationally guided Ala scanning, we identified position 17 of the N-terminal capping repeat to play a key role in overall protein thermostability. The melting temperature of a DARPin domain with a single full-consensus internal repeat was increased by 8...

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.