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Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process.

Protein engineering, design & selection : PEDS - 1 Sept 2015

Houlihan Gillian, Gatti-Lafranconi Pietro, Lowe David, Hollfelder Florian

Abstract excerpt

In vitro display technologies have proved to be powerful tools for obtaining high-affinity protein binders. We recently described SNAP display, an entirely in vitro DNA display system that uses the SNAP-tag to link protein with its encoding DNA in water-in-oil emulsions. Here, we apply SNAP display for the affinity maturation of a designed ankyrin repeat proteins (DARPin) that binds to the extracellular domain of...

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Share to Ankyrin Repeat
Share to Antibodies

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

Share to Mutagenesis, Site-Directed

Share to Protein Structure, Tertiary

Share to Receptor, ErbB-2

Share to Ribosomes

Share to Selection, Genetic

Source

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Identifiers

PMID: 26134501
DOI: 10.1093/protein/gzv029
PMCID: PMC4550541

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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