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Article

A hot-spot motif characterizes the interface between a designed ankyrin-repeat protein and its target ligand.

Biophysical journal - 8 Feb 2012

Cheung Luthur Siu-Lun, Kanwar Manu, Ostermeier Marc, Konstantopoulos Konstantinos

Abstract excerpt

Nonantibody scaffolds such as designed ankyrin repeat proteins (DARPins) can be rapidly engineered to detect diverse target proteins with high specificity and offer an attractive alternative to antibodies. Using molecular simulations, we predicted that the binding interface between DARPin off7 and its ligand (maltose binding protein; MBP) is characterized by a hot-spot motif in which binding energy is largely...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22325262
DOI: 10.1016/j.bpj.2012.01.004
PMCID: PMC3274810

Genes

MBP

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