Article
Functional analysis of AEBP2, a PRC2 Polycomb protein, reveals a Trithorax phenotype in embryonic development and in ESCs.
Development (Cambridge, England) - 1 Aug 2016
Grijzenhout Anne, Godwin Jonathan, Koseki Haruhiko, Gdula Michal Ryszard, Szumska Dorota, McGouran Joanna F, Bhattacharya Shoumo, Kessler Benedikt M, Brockdorff Neil, Cooper Sarah
Abstract excerpt
The Polycomb repressive complexes PRC1 and PRC2 are key mediators of heritable gene silencing in multicellular organisms. Here, we characterise AEBP2, a known PRC2 co-factor which, in vitro, has been shown to stimulate PRC2 activity. We show that AEBP2 localises specifically to PRC2 target loci, including the inactive X chromosome. Proteomic analysis confirms that AEBP2 associates exclusively with PRC2 complexes....
Topics
- Animals
- Cell Line
- DNA-Binding Proteins
- Embryonic Stem Cells
- Female
- Histones
- Mice
- Mutation
- Nuclear Proteins
- Polycomb Repressive Complex 2
- Proteomics
- Repressor Proteins