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Article

Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Marcos-Vadillo Elena, García-Sánchez Asunción

Abstract excerpt

The development of reporters systems has simplified the study of promoter activity in different areas of knowledge, and represents an easy and fast approach to study genetic variations. In this chapter, we show a transfection protocol of A549 lung epithelial cells with a reporter vector, using the Luciferase-Renilla dual system for studying the variations caused by several polymorphisms in the promoter region of...

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PMID: 27300540
DOI: 10.1007/978-1-4939-3652-6_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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