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Article

Streamlined approach to functional analysis of promoter-region polymorphisms.

BioTechniques - 1 Aug 2002

Coleman S L, Hoogendoorn B, Guy C, Smith S K, O'Donovan M C, Buckland P R

Abstract excerpt

We have developed a rapid method for identifying functional promoter-region polymorphisms. Using a modified pGL3 luciferase expression T-vector, we can amplify by PCR, clone, identify allelic pairs of a polymorphic gene promoter region, and prepare plasmids for cell culture 10 promoters (20 allele pairs) per week per researcher. By utilizing 96-well plate technology and an internal control plasmid expressing...

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Identifiers

PMID: 12188194
DOI: 10.2144/02332ht01

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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