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Article

Gene Reporter Assays to Study miRNA Function.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Abel Yoann, Rederstorff Mathieu

Abstract excerpt

This chapter describes one of the most reliable quantitative assays to test the silencing of a possible target gene by a specific miRNA using a luciferase reporter gene. The experimental procedure first consists in cloning both the wild-type and mutated forms of the 3'UTR of the miRNA-predicted mRNA target downstream of a firefly luciferase reporter. Next, each construct is co-transfected together with the miRNA...

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PMID: 33792877
DOI: 10.1007/978-1-0716-1386-3_12

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