Article
Probing the impact of chromatin conformation on genome editing tools.
Nucleic acids research - 27 Jul 2016
Chen Xiaoyu, Rinsma Marrit, Janssen Josephine M, Liu Jin, Maggio Ignazio, Gonçalves Manuel A F V
Abstract excerpt
Transcription activator-like effector nucleases (TALENs) and RNA-guided nucleases derived from clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 systems have become ubiquitous genome editing tools. Despite this, the impact that distinct high-order chromatin conformations have on these sequence-specific designer nucleases is, presently, ill-defined. The same applies to the relative...
Topics
- CRISPR-Cas Systems
- Chromatin
- Epigenesis, Genetic
- Gene Editing
- Genetic Engineering
- Genotype
- Humans
- Molecular Conformation
- Streptococcus pyogenes
- Transcription Activator-Like Effector Nucleases