Article
Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).
Nature structural & molecular biology - 1 Feb 2018
Maass Philipp G, Barutcu A Rasim, Shechner David M, Weiner Catherine L, Melé Marta, Rinn John L
Abstract excerpt
Imaging and chromatin capture techniques have provided important insights into our understanding of nuclear organization. A limitation of these techniques is the inability to resolve allele-specific spatiotemporal properties of genomic loci in living cells. Here, we describe an allele-specific CRISPR live-cell DNA imaging technique (SNP-CLING) to provide the first comprehensive insights into allelic positioning...
Topics
- Alleles
- Animals
- Apoptosis
- Cell Nucleolus
- Chondrocytes
- Clustered Regularly Interspaced Short Palindromic Repeats
- Female
- Fibroblasts
- Humans
- Male
- Mice
- Microscopy, Confocal
- Mouse Embryonic Stem Cells
- Polymorphism, Single Nucleotide