Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Selection of affinity-improved neutralizing human scFv against HBV PreS1 from CDR3 VH/VL mutant library.

Biologicals : journal of the International Association of Biological Standardization - 1 Jul 2016

Chen YanMin, Bai Yin, Guo XiaoChen, Wang WenFei, Zheng Qi, Wang FuXiang, Sun Dejun, Li DeShan, Ren GuiPing, Yin JieChao

Abstract excerpt

A CDR3 mutant library was constructed from a previously isolated anti-HBV neutralizing Homo sapiens scFv-31 template by random mutant primers PCR. Then the library was displayed on the inner membrane surface in Escherichia coli periplasmic space. Seven scFv clones were isolated from the mutant library through three rounds of screening by flow cytometry. Competition ELISA assay indicates that isolated scFv...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibody Affinity
Share to Antibody Specificity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Complementarity Determining Regions

Share to Hep G2 Cells

Share to Hepatitis B Antibodies

Share to Immunoglobulin Light Chains

Share to Peptide Library

Share to Single-Chain Antibodies

Source

Identifiers

PMID: 27255707
DOI: 10.1016/j.biologicals.2016.05.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Selection of affinity-improved neutralizing human scFv against HBV PreS1 from CDR3 VH/VL mutant library.PMID 27255707Read ↗

Improvement of neutralizing activity of human scFv antibodies against hepatitis B virus binding using CDR3 V(H) mutant library.Semantic proximity Read ↗

A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C.Semantic proximity Read ↗

A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity.Semantic proximity Read ↗

Isolation and characterization of a novel human scFv inhibiting EGFR vIII expressing cancers.Semantic proximity Read ↗

Construction and Characterization of an Anti-Hepatitis B Virus preS1 Humanized Antibody that Binds to the Essential Receptor Binding Site.Semantic proximity Read ↗

By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selectionSemantic proximity Read ↗

Single Chain Fragment Variable (scFv) corresponding to a novel epitope within HCV envelope protein restricts virus entry into hepatocytesSemantic proximity Read ↗

Construction of a Large Synthetic Human scFv Library with Six Diversified CDRs and High Functional DiversitySemantic proximity Read ↗

Optimization and modification of anti-rhTNF-α single chain variable fragment antibody: effective in vitro affinity maturation and functional expression of chimeric Fab.Semantic proximity Read ↗

Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism.Semantic proximity Read ↗

Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library.Semantic proximity Read ↗

Affinity maturation to improve human monoclonal antibody neutralization potency and breadth against hepatitis C virus.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.