Article
Optimization and modification of anti-rhTNF-α single chain variable fragment antibody: effective in vitro affinity maturation and functional expression of chimeric Fab.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie - 1 Jun 2013
Miao Xiaoniu, Li Andong, Chen Wei, Qi Haidi, Qiu Zhen, Zhang Yubin, Zhang Juan, Wang Min
Abstract excerpt
AIMS: Single chain variable fragment (scFv) is one of the most popular recombinant antibody (rAb) formats. However, sometimes scFv with the most favorable specificity profile lack sufficient affinity or acceptable pharmacokinetics for clinical applications. To address these problems, we described a method to modify recombinant anti-rhTNF-α scFv-F6D2E7. RESULTS: Random mutations were inserted into CDR-H3 by...
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