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Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library.

Molecular biology reports - 1 Apr 2010

Koohapitagtam Maneerat, Rungpragayphan Suang, Hongprayoon Ratchanee, Kositratana Wichai, Sirinarumitr Theerapol

Abstract excerpt

Non-immune phage scFv library is one of the most attractive resources for therapeutics, diagnostics and basic research. As a matter of fact, quality of the library is limited by inefficient PCR cloning of antibody genes using degenerated primers. PCR using this type of primers is difficult to optimize conditions for efficient amplification, and therefore causes loss of antibody diversities. To overcome this...

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Share to Cloning, Molecular
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Electrophoresis, Polyacrylamide Gel

Share to Immunoglobulin Heavy Chains

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Share to Immunoglobulin Variable Region

Share to Peptide Library

Share to Transformation, Genetic

Source

Identifiers

PMID: 19554473
DOI: 10.1007/s11033-009-9583-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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