Article
A new method for quantifying mitochondrial axonal transport.
Protein & cell - 1 Nov 2016
Chen Mengmeng, Li Yang, Yang Mengxue, Chen Xiaoping, Chen Yemeng, Yang Fan, Lu Sheng, Yao Shengyu, Zhou Timothy, Liu Jianghong, Zhu Li, Du Sidan, Wu Jane Y
Abstract excerpt
Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named "MitoQuant". This tool-kit consists of...
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