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Article

A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.

Scientific reports - 10 May 2016

Williams Michael R, Fricano-Kugler Catherine J, Getz Stephanie A, Skelton Patrick D, Lee Jeonghoon, Rizzuto Christian P, Geller Joseph S, Li Meijie, Luikart Bryan W

Abstract excerpt

Retroviruses expressing a fluorescent protein, Cas9, and a small guide RNA are used to mimic nonsense PTEN mutations from autism patients in developing mouse neurons. We compare the cellular phenotype elicited by CRISPR-Cas9 to those elicited using shRNA or Cre/Lox technologies and find that knockdown or knockout (KO) produced a corresponding moderate or severe neuronal hypertrophy in all cells. In contrast, the...

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Source

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Identifiers

PMID: 27161796
DOI: 10.1038/srep25611
PMCID: PMC4861960

Genes

KATNAL2
PTEN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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