Article
DNA compaction by the bacteriophage protein Cox studied on the single DNA molecule level using nanofluidic channels.
Nucleic acids research - 6 Sept 2016
Frykholm Karolin, Berntsson Ronnie Per-Arne, Claesson Magnus, de Battice Laura, Odegrip Richard, Stenmark Pål, Westerlund Fredrik
Abstract excerpt
The Cox protein from bacteriophage P2 forms oligomeric filaments and it has been proposed that DNA can be wound up around these filaments, similar to how histones condense DNA. We here use fluorescence microscopy to study single DNA-Cox complexes in nanofluidic channels and compare how the Cox homologs from phages P2 and WΦ affect DNA. By measuring the extension of nanoconfined DNA in absence and presence of Cox...
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