Article
Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy.
The EMBO journal - 17 Mar 2003
Orlova Elena V, Gowen Brent, Dröge Anja, Stiege Asita, Weise Frank, Lurz Rudi, van Heel Marin, Tavares Paulo
Abstract excerpt
In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the connector structure from bacteriophage SPP1 using cryo-electron microscopy and single particle analysis. The multiprotein complex comprises the portal protein gp6 and the head completion proteins gp15 and gp16. Although...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacillus Phages
- Bacillus subtilis
- Capsid
- Cryoelectron Microscopy
- DNA, Viral
- Microscopy, Immunoelectron
- Models, Molecular
- Mutation
- Protein Conformation