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Article

Role of Internal DNA Motion on the Mobility of a Nucleoid-Associated Protein.

The journal of physical chemistry letters - 1 Oct 2020

Yadav Indresh, Basak Rajib, Yan Peiyan, van Kan Jeroen A, Arluison Véronique, van der Maarel Johan R C

Abstract excerpt

Protein transport on DNA is at the core of the machinery of life. Here we investigated the influence of DNA internal motion on the mobility of Hfq, which is involved in several aspects of nucleic acid metabolism and is one of the nucleoid-associated proteins that shape the bacterial chromosome. Fluorescence microscopy was used to follow Hfq on double-stranded DNA that was stretched by confinement to a channel...

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PMID: 32930601
DOI: 10.1021/acs.jpclett.0c02251

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