Article
Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variant.
Scientific reports - 1 Jan 2013
Piatkevich Kiryl D, Malashkevich Vladimir N, Morozova Kateryna S, Nemkovich Nicolai A, Almo Steven C, Verkhusha Vladislav V
Abstract excerpt
Most GFP-like fluorescent proteins exhibit small Stokes shifts (10-45 nm) due to rigidity of the chromophore environment that excludes non-fluorescent relaxation to a ground state. An unusual near-infrared derivative of the red fluorescent protein mKate, named TagRFP675, exhibits the Stokes shift, which is 30 nm extended comparing to that of the parental protein. In physiological conditions, TagRFP675 absorbs at...
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