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Directed evolution of a β-glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars.

Protein engineering, design & selection : PEDS - 1 Sept 2012

Shim Jae-Hoon, Chen Hong-Ming, Rich Jamie R, Goddard-Borger Ethan D, Withers Stephen G

Abstract excerpt

Glycans bearing modified hydroxyl groups are common in biology but because these modifications are added after assembly, enzymes are not available for the transfer and coupling of hydroxyl-modified monosaccharide units. Access to such enzymes could be valuable, particularly if they can also introduce 'bio-orthogonal tags'. Glycosynthases, mutant glycosidases that synthesize glycosides using glycosyl fluoride...

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PMID: 22893693
DOI: 10.1093/protein/gzs045

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