Article
Characterization of the glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides and oligosaccharides with altered linkage composition.
Food chemistry - 15 Feb 2017
Meng Xiangfeng, Pijning Tjaard, Tietema Martin, Dobruchowska Justyna M, Yin Huifang, Gerwig Gerrit J, Kralj Slavko, Dijkhuizen Lubbert
Abstract excerpt
Exopolysaccharides produced by lactic acid bacteria are extensively used for food applications. Glucansucrase enzymes of lactic acid bacteria use sucrose to catalyze the synthesis of α-glucans with different linkage compositions, size and physico-chemical properties. Crystallographic studies of GTF180-ΔN show that at the acceptor binding sites +1 and +2, residue W1065 provides stacking interactions to the...
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