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Article

Structural characterization of bioengineered alpha-D-glucans produced by mutant glucansucrase GTF180 enzymes of Lactobacillus reuteri strain 180.

Biomacromolecules - 9 Mar 2009

van Leeuwen Sander S, Kralj Slavko, Eeuwema Wieger, Gerwig Gerrit J, Dijkhuizen Lubbert, Kamerling Johannis P

Abstract excerpt

Mutagenesis of specific amino acid residues of the glucansucrase (GTF180) enzyme from Lactobacillus reuteri strain 180 yielded 12 mutant enzymes that produced modified exopolysaccharides (mEPSs) from sucrose. Ethanol-precipitated and purified mEPSs were subjected to linkage analysis, Smith degradation analysis, and 1D/2D (1)H NMR spectroscopy. Comparison of the results with structural data of the previously...

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Identifiers

PMID: 19186951
DOI: 10.1021/bm801240r

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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