Article
Synthesis of New Hyperbranched α-Glucans from Sucrose by Lactobacillus reuteri 180 Glucansucrase Mutants.
Journal of agricultural and food chemistry - 20 Jan 2016
Meng Xiangfeng, Dobruchowska Justyna M, Pijning Tjaard, Gerwig Gerrit J, Dijkhuizen Lubbert
Abstract excerpt
α-Glucans produced by glucansucrase enzymes of lactic acid bacteria attract strong attention as novel ingredients and functional biopolymers in the food industry. In the present study, α-helix 4 amino acid residues D1085, R1088, and N1089 of glucansucrase GTF180 of Lactobacillus reuteri 180 were targeted for mutagenesis both jointly and separately. Analysis of the mutational effects on enzyme function revealed...
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