Transglycosylation of engineered cyclodextrin glucanotransferases as O-glycoligases.

Abstract excerpt

An O-glycoligase is a hydrolytically impaired mutant of a retaining α-glycosidase in which the catalytic acid/base has been removed, but which can still perform transglycosylation when incubated with activated glycosyl fluoride donor sugars. In this paper, we describe another example, wherein a cyclodextrin glucanotransferase mutant (CGT-E284A) with an alanine residue at its general acid/base catalyst position...

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