Article
PCR amplification of specific alleles: rapid detection of known mutations and polymorphisms.
Mutation research - 1 Jul 1993
Bottema C D, Sommer S S
Abstract excerpt
We review a method termed PCR Amplification of Specific Alleles (PASA), a generally applicable technique for the detection of known point mutations, small deletions and insertions, polymorphisms and other sequence variations. PASA is a modification of PCR that depends on the synthesis of a PCR oligonucleotide primer that precisely matches with one of the alleles but mismatches with the other. When the mismatch...
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