Article
PCR amplification of specific alleles: rapid detection of known mutations and polymorphisms.
Mutation research - 1 Jul 1993
Bottema C D, Sommer S S
Abstract excerpt
We review a method termed PCR Amplification of Specific Alleles (PASA), a generally applicable technique for the detection of known point mutations, small deletions and insertions, polymorphisms and other sequence variations. PASA is a modification of PCR that depends on the synthesis of a PCR ol...
Topics
- Alleles
- DNA Mutational Analysis
- DNA, Single-Stranded
- DNA-Directed DNA Polymerase
- Factor IX
- Female
- Genetic Carrier Screening
- Hot Temperature
- Humans
- Magnesium
- Male
- Nucleic Acid Heteroduplexes
- Oligonucleotides
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Reproducibility of Results
- Taq Polymerase