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Simplifying Genotyping of Mutants from Genome Editing with a Parallel qPCR-Based iGenotype Index.

Cells - 29 Jan 2024

Fu Liezhen, Wang Shouhong, Liu Lusha, Shibata Yuki, Okada Morihiro, Luu Nga, Shi Yun-Bo

Abstract excerpt

Targeted genome editing is a powerful tool in reverse genetic studies of gene function in many aspects of biological and pathological processes. The CRISPR/Cas system or engineered endonucleases such as ZFNs and TALENs are the most widely used genome editing tools that are introduced into cells or fertilized eggs to generate double-strand DNA breaks within the targeted region, triggering cellular DNA repair...

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PMID: 38334640
DOI: 10.3390/cells13030247
PMCID: PMC10854663

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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