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Article

Allele-specific PCR in SNP genotyping.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Gaudet Muriel, Fara Anna-Giulia, Beritognolo Isacco, Sabatti Maurizio

Abstract excerpt

The increasing need for large-scale genotyping applications of single nucleotide polymorphisms (SNPs) in model and nonmodel organisms requires the development of low-cost technologies accessible to minimally equipped laboratories. The method presented here allows efficient discrimination of SNPs by allele-specific PCR in a single reaction with standard PCR conditions. A common reverse primer and two forward...

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PMID: 19768609
DOI: 10.1007/978-1-60327-411-1_26

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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