Article
Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9.
Nature - 5 May 2016
Paquet Dominik, Kwart Dylan, Chen Antonia, Sproul Andrew, Jacob Samson, Teo Shaun, Olsen Kimberly Moore, Gregg Andrew, Noggle Scott, Tessier-Lavigne Marc
Abstract excerpt
The bacterial CRISPR/Cas9 system allows sequence-specific gene editing in many organisms and holds promise as a tool to generate models of human diseases, for example, in human pluripotent stem cells. CRISPR/Cas9 introduces targeted double-stranded breaks (DSBs) with high efficiency, which are typically repaired by non-homologous end-joining (NHEJ) resulting in nonspecific insertions, deletions or other mutations...
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