Article
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice.
Nature communications - 26 Feb 2016
Maresch Roman, Mueller Sebastian, Veltkamp Christian, Öllinger Rupert, Friedrich Mathias, Heid Irina, Steiger Katja, Weber Julia, Engleitner Thomas, Barenboim Maxim, Klein Sabine, Louzada Sandra, Banerjee Ruby, Strong Alexander, Stauber Teresa, Gross Nina, Geumann Ulf, Lange Sebastian, Ringelhan Marc, Varela Ignacio, Unger Kristian, Yang Fengtang, Schmid Roland M, Vassiliou George S, Braren Rickmer, Schneider Günter, Heikenwalder Mathias, Bradley Allan, Saur Dieter, Rad Roland
Abstract excerpt
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery of CRISPR/Cas9 to the pancreas of adult mice, allowing simultaneous editing of multiple gene sets in individual cells. We use the method to induce pancreatic cancer and exploit CRISPR/Cas9 mutational signatures for...
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