Article
KRAS Engages AGO2 to Enhance Cellular Transformation.
Cell reports - 16 Feb 2016
Shankar Sunita, Pitchiaya Sethuramasundaram, Malik Rohit, Kothari Vishal, Hosono Yasuyuki, Yocum Anastasia K, Gundlapalli Harika, White Yasmine, Firestone Ari, Cao Xuhong, Dhanasekaran Saravana M, Stuckey Jeanne A, Bollag Gideon, Shannon Kevin, Walter Nils G, Kumar-Sinha Chandan, Chinnaiyan Arul M
Abstract excerpt
Oncogenic mutations in RAS provide a compelling yet intractable therapeutic target. Using co-immunoprecipitation mass spectrometry, we uncovered an interaction between RAS and Argonaute 2 (AGO2). Endogenously, RAS and AGO2 co-sediment and co-localize in the endoplasmic reticulum. The AGO2 N-terminal domain directly binds the Switch II region of KRAS, agnostic of nucleotide (GDP/GTP) binding. Functionally, AGO2...
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