Article
A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimens.
British journal of cancer - 10 Jul 2012
Gonzalez de Castro D, Angulo B, Gomez B, Mair D, Martinez R, Suarez-Gauthier A, Shieh F, Velez M, Brophy V H, Lawrence H J, Lopez-Rios F
Abstract excerpt
BACKGROUND: KRAS mutation testing is required to select patients with metastatic colorectal cancer (CRC) to receive anti-epidermal growth factor receptor antibodies, but the optimal KRAS mutation test method is uncertain. METHODS: We conducted a two-site comparison of two commercial KRAS mutation kits - the cobas KRAS Mutation Test and the Qiagen therascreen KRAS Kit - and Sanger sequencing. A panel of 120 CRC...
Topics
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms
- Female
- Formaldehyde
- Humans
- Male
- Middle Aged
- Mutation
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins p21(ras)
- Reproducibility of Results